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Journal: The Journal of Clinical Investigation
Article Title: Leukemia-expanded splenic CD81 + erythroblasts potentiate disease progression in mice by reshaping leukemic cell metabolism
doi: 10.1172/JCI193082
Figure Lengend Snippet: ( A ) A schematic illustrating the experimental design: CD45 − CD71 + TER119 + CD44 + erythroblasts (total Erys) from spleens of CTRL mice or mice with advanced AML were sorted by flow cytometry and processed for scRNA-Seq on the 10x Genomics platform. ( B ) UMAP plot showing the cell cluster distribution for AML Erys and CTRL Erys. ( C ) Top 5 gene ontology terms and their corresponding P values in the identified clusters. ( D ) Pseudotime analysis illustrating the differentiation trajectory of C1–C4 (top) and the distribution of each cluster (bottom). ( E ) Relative proportions of AML Erys (red) and CTRL Erys (blue) in each cluster. ( F and G ) Directional network plots showing the number of incoming ( F ) and outgoing ( G ) interactions for AML C1 Erys and other cells in the AML microenvironment, including AML cells, endothelial cells (ECs), pericytes, Tcf21 + fibroblasts, and Tcf21 + mesenchymal stem cells (MSCs). ( H ) Venn diagram illustrating the intersection of C1 signature genes and a list of surface marker genes . The 14 identified genes are listed in the box below the Venn diagram. ( I ) Expression levels of the top 5 C1 surface marker genes across C1–C4. ( J ) Expression of Cd81 in C1 versus in C2–C4. ( K ) Representative Wright-Giemsa staining of the sorted CD81 + Erys and CD81 − Erys from the spleens of AML mice. Scale bar: 20 μm. ( L ) Cell counts and frequencies of CD81 + Erys in the spleens of CTRL mice and mice at different stages of AML ( n = 4). Data are presented as the mean ± SEM. The results are representative of 1 of 3 independent experiments with consistent trends. * P < 0.05 and *** P < 0.001, by 1-way ANOVA ( L ).
Article Snippet: To determine the function of AML-expanded C1 Erys, we next intersected the signature genes of C1 with a curated
Techniques: Flow Cytometry, Marker, Expressing, Staining